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Objectives in terms of knowledge (course content) :

  • Reminders on transcription and translation
  • History of Molecular Biology
  • Restriction enzymes and modifying enzymes
  • Cloning vectors - Phages lambda and M13 - Prokaryotic and eukaryotic plasmid vectors
  • Molecular hybridization
  • Expression librairies-subtractive librairies
  • PCR and qPCR
  • Techniques of site-specific mutagenesis.
  • RNA interference-DNA interference (Talens-CRISPR / Cas9)
  • New sequencing techniques
  • Recombinant cloning

Compétences visées

Objectives in terms of skills :

  • This course aims to present the different techniques of cloning, manipulation and analysis of nucleic acid sequences. All of these topics will be treated in an integrated manner to enable students to become familiar with many cloning strategies, and directed mutagenesis
  • Strategies for cloning and modifying DNA sequences - Using different cloning and sequence analysis software


Bibliographie, support du cours :

  • Fichier Power Point sur Moodle (Français et Anglais)
  • Biochimie Générale JH Weil 11éme Edition Dunod Sciences
  • Genes (Eds. Wiley)


Responsable(s) de l'enseignement
Bruno Chatton :