EC
TP Génie génétique et microbiologie
Description
Objectives in terms of knowledge (course content) :
Preparation of materials
- Agar plates w/ antibiotics
- Agarose gels
- Competent cells
Experimentation
- Miniprep/Midiprep
- Cloning of GFP into pUC19 (digestion, PCR, gel excision, ligation, transformation, colony picking)
- Cloning of sgRNA into PX459 (digestion, annealing oligos, ligation, transformation, colony picking)
- Verification of cloning by restriction digest
- Colony PCR
- RNA extraction
- Reverse transcription
- qPCR
Analysis
- Primer selection
- Enzyme selection
- Clonings
- qPCR results
Compétences visées
Objectives in terms of skills:
- Understanding the protocols used in genetic engineering
- Acquiring the theoretical bases of cloning
- Acquiring a good practice in genetic engineering
- Being able to analyze and discuss experimental results
- Writting of a lab book
- Cleaning of the room and machines used
Bibliographie
Protocols and documents necessary to perform the experiments are given before the practical and all documents are available on Moodle.