UE
Advanced spectroscopic methods
Description
· Electronic Spectrophotometry: Historical recalls; Molecular orbitals; Far UV and visible; Conditions to accurately acquire and rationalized electronic transitions; electronic transitions in solution: auxochrome effects and Charge transfer band, Analysis; Instrumentation.
· Emission Spectrophotometry: Different luminescence processes; radiative and non radiative processes; photophysical properties; Collision and static quenching, Development of novel fluorescent probes: The case of Iron; Instrumentation.
· Infra Red and Raman spectrophotometry: Instrumentation: choice, advantages and limitations; Molecular vibrational and rotational theory; Recalls on the group theory and interpretation of spectra; Examples; Instrumentation.
· Diffusion Raman Spectrophotometry: Principle of the method; Surface Exaltation; Instrumentation.
· Solid state NMR spectroscopy: Dipolar and quadrupolar coupling, interaction anisotropy, Magic angle spinning, static/orientated samples, some experimental NMR pulse sequences, Applications
Compétences visées
· Understand the ground rules of several complementary spectroscopic techniques to characterize various systems in solution.
· Identify the importance and advantages of each of spectroscopy studied in a given scientific area.
· Being able, at the end of the teaching, to explain the principles of these spectroscopic techniques and the observations and phenomena that are associated with them.
Compétences développées et/ou évaluées
· Understanding the investigated spectroscopic approaches for the characterization and the quantification of (bio)molecules or metal complexes.
· Being able to apply these spectroscopic techniques and principles to any molecular systems in solution.