EC
Enzymology practicals
Description
Objectives for knowledge :
- Understanding and practicing enzymology basics including:
- Enzymatic activity
- Kinetics of enzymatic activity
- Immobilizing enzymes, a lecture and practice at small and medium scales
- The beta-galactosidase as a prototypic enzyme
- Hydrolysis of lactose, purified and contained in milk
- Discussions on the strategies and results
Compétences visées
Objectives in terms of skills :
- Acquire a good practice for work with proteins including enzymes, with kinetics
- Using spectrophotometers
- Acquire the notion of making appropriate graphs, of experimental variability/reproducibility
- Be able to relate the theory of enzymology to experimental results
- Be able to analyze and discuss on experimental results
- Be able to present experimental results