EC
Plant molecular biology practicals
Description
Learning objectives (course content) :
- Plant hormones and mobilization of starch reserves in germinating grain
- Plant cell culture and plantlet regeneration
- Genetic engineering of seed content
- Induced resistance in wine grape and resveratrol production
- Transcriptomics and proteomics of stress responses in moss
- Transient expression of recombinant proteins in plant protoplasts
- In vivo calcium measurements
- Microinjection of plant cells
- Genetics of photoreceptors in plants
- Transformation of plants by particle bombardment and Agrobacterium infection
- Genetics of stem cell formation and maintenance in plants
- Epigenetic imprinting and reciprocal crosses
- Gene regulation by micro-RNAs
- Mapping a point mutation in Arabidopsis thaliana
Compétences visées
Objectives in terms of acquired skills :
(ie roughly what the student should be able to understand and apply in practical situations at the end of the course)
- To familiarize the student with genetics, physiology, genetic engineering and cell biology of plants.
- To foster scientific communication through seminars, critical review of literature and writing reports of experiments conducted during the course.
Bibliographie
References, books, course support :
- Lab manual of 220 pages.
- Scientific review and research articles.
- Lecture, tutorial and discussion periods