Enzymology practicals

Description

Objectives for knowledge :

• Understanding and practicing enzymology basics including:
• Enzymatic activity
• Kinetics of enzymatic activity
• Immobilizing enzymes, a lecture and practice at small and medium scales
• The beta-galactosidase as a prototypic enzyme
• Hydrolysis of lactose, purified and contained in milk
• Discussions on the strategies and results

Compétences visées

Objectives in terms of skills :

• Acquire a good practice for work with proteins including enzymes, with kinetics
• Using spectrophotometers
• Acquire the notion of making appropriate graphs, of experimental variability/reproducibility
• Be able to relate the theory of enzymology to experimental results
• Be able to analyze and discuss on experimental results
• Be able to present experimental results

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